Using circulating cell-free DNA to monitor personalized cancer therapy

High-quality genomic analysis is critical for personalized pharmacotherapy in patients with cancer

By | April 25th, 2017|Other publications|0 Comments

Dr hab. n. med. Jakub Dobruch, FEBU – Liquid Biopsy

Dr hab. n. med. Jakub Dobruch, FEBU. Urological Review, The Polish Association of Urology bimonthly magazine. No. 5/2016

Liquid Biopsy is an example of a new kind of research that could revolutionize cancer detection and treatment of patients affected.

By | November 14th, 2016|Other publications|0 Comments

Prof. Piotr Radziszewski – New generation oncological biomarkers – focusing on Chronix company

Lecture at the 5th Polish women and men health debate according to Medical guidelines – kidney cancer, bladder cancer.

By | November 3rd, 2016|Other publications, Video|0 Comments

Predicting Treatment Outcomes in Immunotherapy

Weiss, G. et al. Tumor cell-free DNA copy number instability (CNI) to predict therapeutic response to immunotherapy prior to cycle 2. J ClinOncol 34, 2016 (suppl; abstr 3027).

We measured CNAs changes during treatment by computing a genomic copy number instability index (CNI) of cfDNA and Treg-specific demethylation region (TSDR) as measure for Tregs% of leukocytes compared to response.

By | November 3rd, 2016|Other publications|0 Comments

Minimal Residual Disease in Breast Cancer

Urnovitz, HB et al. Modulation of breast cancer cell-free DNA with surgical resection. J ClinOncol 31, 2013 (suppl; abstr 11060).

In this study, we sought to determine whether cfDNA decreases after surgery in patients with operable invasive breast cancer. Serum was collected before and 1-4 weeks after surgery from 16 breast cancer patients and also from 24 age and gender matched controls.

By | November 3rd, 2016|Other publications|0 Comments

Breast Cancer cfDNA with NGS

Beck, J. et al. Next Generation Sequencing of Serum Circulating Nucleic Acids from Invasive Ductal Breast Cancer Patients Reveals Differences to Healthy and Non-malignant Controls. Mol. Cancer Res. 2010; 8: 385-92.

Circulating nucleic acids (CNA) isolated from serum or plasma are increasingly recognized as biomarkers for cancers.We report specific DNA-transposable element sequences that could discriminate all stages of invasive ductal breast carcinoma with significant specificity and sensitivity.

By | November 3rd, 2016|Other publications|0 Comments

Liquid biopsies in predicting and monitoring response to therapy in advanced pancreatic cancer

Madappa N. Kundranda Genomic change index (GCI) and liquid biopsies in predicting and monitoring response to therapy in advanced pancreatic ductal adenocarcinoma (PDAC). J Clin Oncol 33, 2015 (suppl 3; abstr 309).

Advanced PDAC is a lethal disease with dismal 5 year survival. The current modalities for monitoring therapy response using serum (CA) 19-9 levels have poor specificity and radiological responses have long lag times. Tests for rapid and accurate clinical assessment are needed for early decision making.

By | November 2nd, 2016|Other publications|0 Comments

Prognostic value of cell-free DNA in patients with oropharyngeal cancers.

Julia Beck, Margret Rave-Fraenk, et al. Prognostic value of cell-free DNA in patients with oropharyngeal cancers.J Clin Oncol 34, 2016 (suppl; abstr e17511).

Tumor derived plasma cell-free DNA (TcfDNA) is described as biomarker to monitor tumor burden in cancer. Large somatic genome aberrations are hallmarks of malignancies and detectable in cfDNA. However, levels of TcfDNA are variable according to cancer types. We obtained copy-number instability (CNI) scores of cfDNA in treatment-naïve head and neck cancers before and after therapy.

By | November 2nd, 2016|Other publications|0 Comments

Combining Genome Change Index (GCI) and liquid biopsy to predict and monitor therapeutic responses.

Ekkehard Schütz, Julia Beck, Donald Peter Braun, Howard B. Urnovitz , et al. Combining Genome Change Index (GCI) and liquid biopsy to predict and monitor therapeutic responses.J Clin Oncol 33, 2015 (suppl; abstr e22020) .

Genomic instability of tumor cells has been associated with a poor prognosis. However, impaired DNA repair pathways leading to genomic instability are also described to increase tumor sensitivity to DNA damaging agents. A comprehensive Genomic Change Index (GCI) as an indicator of defective DNA repair is proposed as a potential predictor of cytotoxic chemotherapy (chemo) response.

By | November 2nd, 2016|Other publications|0 Comments

Quantifying copy number variations in cell-free DNA for potential clinical utility from a large prostate cancer cohort.

Ekkehard Schütz, Howard B. Urnovitz, et al. Quantifying copy number variations in cell-free DNA for potential clinical utility from a large prostate cancer cohort J Clin Oncol 31, 2013 (suppl; abstr 5072).

Prostate cancer (PrCa) is the most frequent non-dermatological malignancy in the male population. Genomic instability resulting in copy number variation (CNV) is a hallmark of malignant transformation.

By | November 2nd, 2016|Other publications|0 Comments